Article No
AFC-0264UZ-0.1
Anti-Hu CD79a Purified
Anti-Hu CD79a Purified
Antibodies Primary
Specifications
| Application | FC, IP, WB, IHC |
| Article No | AFC-0264UZ-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | HM47 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD79a Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 973 |
| Format | Purified |
| Immunogen | Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a |
| Isotype | IgG1 k |
| Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
| Notes | CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling. |
| Alias Names | BCR alpha, Ig-alpha, MB-1, IGA |
| Previous Article No | AFC-4572-2, AFC-0264UZ-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | dog/canine, chicken, rabbit, guinea pig, horse/equine, cow/bovine, mouse, rat, pig/porcine, monkey, human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms. |
| UniProt Number | P11912 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
