Article No
AFC-OE0258-0.1
Application | FC, IP, IHC, ICC |
Article No | AFC-OE0258-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | E11 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD35 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 1378 |
Format | Purified |
Immunogen | Acute monocytic leukemia cells and normal blood monocytes |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production. |
Alias Names | CR1, KN, C3BR, C4BR |
Previous Article No | AFC-4551-2, AFC-OE0258-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval. Immunohistochemistry (frozen sections): Acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | monkey, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes. |
UniProt Number | P17927 |
Product Page Updated | 2024-01-11T15:10:38.882Z |