Article No
AFC-6NF7NL-0.1
Mouse IgG2b Isotype Control Purified
Mouse IgG2b Isotype Control Purified
Antibodies Isotype Control
Specifications
| Application | ELISA, FC, IP, WB, IHC, ICC |
| Article No | AFC-6NF7NL-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MPC-11 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Mouse IgG2b Isotype Control Purified |
| Supplier | Nordic Biosite |
| Format | Purified |
| Immunogen | KLH-coupled trinitrophenol |
| Isotype | IgG2b k |
| Keywords | Immunoglobulin Isotype Controls |
| Notes | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Previous Article No | AFC-4540-2, AFC-6NF7NL-0.1 |
| Product Type | Antibodies Isotype Control |
| Protocol | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 mg |
| Source / Host | mouse |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues. |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
