Article No
AFC-WMZ2RP-0.1
Application | FC, IHC, ICC, ELISA |
Article No | AFC-WMZ2RP-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MAb11 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-TNF alpha Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 7124 |
Format | Purified |
Immunogen | Recombinant human TNF-alpha |
Isotype | IgG1 k |
Keywords | Cytokine Signaling (Human) |
Notes | TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis. |
Alias Names | TNFA, DIF, TNLG1F |
Previous Article No | AFC-4536-2, AFC-WMZ2RP-0.1 |
Product Type | Antibodies Primary |
Protocol | ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow cytometry: Intracellular staining. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Cell Signaling |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | pig/porcine, human, monkey |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha). |
UniProt Number | P01375 |
Product Page Updated | 2024-01-03T12:18:20.318Z |