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Anti-Rt CD8b Purified

Antibodies Primary

Make a customized order

Article No

AFC-FCQOXI-0.1

Species Reactivity

rat

Size

0.1 mg

Clone

341

Source / Host

mouse

Application

FC, IP, WB, IHC

Article No

AFC-FCQOXI-0.1

Species Reactivity

rat

Size

0.1 mg

Clone

341

Source / Host

mouse

Application

FC, IP, WB, IHC

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB, IHC
Article No	AFC-FCQOXI-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	341
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Rt CD8b Purified
Supplier	Nordic Biosite
Entrez Gene ID	926
Format	Purified
Immunogen	CD8 positive Wistar rat splenic T cell hybridomas
Isotype	IgG1 k
Keywords	CD and Related Antigens & MHC (Rodent)
Notes	The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Alias Names	Ly-3, Ly-C, Lyt-3
Previous Article No	AFC-4509-2, AFC-FCQOXI-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 1.5 μg/ml.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	rat
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
UniProt Number	P10966
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623,*Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942,*Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15;167(10):5935-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11698471,*Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat. Diabetes. 1999 May;48(5):975-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/10331400,*Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1;161(11):6265-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9834115,*Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160(12):6004-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/9637515,*Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123,*Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153(3):979-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/8027567,*Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov;84(5):1287-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/18682578,,,

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