MRCK alpha

Article No AFC-4492-2
Product Type Antibodies Primary
Research Area Cell Proliferation
Application WB, ICC
Size 0.1 mg


Country Availability all
BioSite Brand BioSite Flow
Clone Polyclonal
Clone Type polyclonal
Concentration 0.25 mg/ml
Conjugation Unconjugated
Description Rabbit Polyclonal to MRCK alpha
Supplier BioSite Flow
Immunogen Peptide corresponding to the amino acids 1671-1685 of human MRCK alpha
Keywords Cytoskeleton: Actin System (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human)
Notes MRCK alpha (myotonic dystrophy kinase-related Cdc42-binding kinase alpha) is a member of the dystrophia myotonica protein kinase (DMPK) family that functions downstream of Cdc42 in actin cytoskeletal reorganization. It is a serine/threonine kinase with multiple functional domains, including phorbol ester-responsive C1 domain. Three independent coiled-coil domains and the N-terminal region preceding the kinase domain are responsible for intermolecular interactions leading to MRCK alpha multimerization. The transautophosphorylation process is critical for regulation of MRCK alpha catalytic activities. Binding of phorbol esters to MRCK releases its autoinhibition, allowing N-terminal dimerization and subsequent kinase activation.
Purification Purified from rabbit serum by affinity chromatography
Purity > 95% (by SDS-PAGE) before adding BSA
Species Reactivity human
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
Technical Specifications The polyclonal antibody anti-MRCK alpha recognizes myotonic dystrophy kinase-related Cdc42-binding kinase alpha, a 180 kDa Ser/Thr kinase, which is responsible for reorganization of actin cytoskeleton.
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

human human

Suggested protocols:

Western Blot

Western Blot