LIME (mouse)

Article No AFC-4479-2
Product Type Antibodies Primary
Research Area Immunology
Application IP, WB
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone mLIME-05
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Mouse Monoclonal to LIME (mouse)
Supplier BioSite Flow
Immunogen Recombinant protein corresponding to amino acids 86-202 of mouse LIME_x000D_
Isotype IgG2a
Keywords Adaptor Proteins (Rodent)
Notes LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. _x000D_
Purification Purified by protein-A affinity chromatography
Purity > 95% (by SDS-PAGE)
Species Reactivity mouse
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Technical Specifications Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells._x000D_
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

mouse mouse