Article No
AFC-GA76GW-0.1
Application | FC, IP, IHC, ELISA |
Article No | AFC-GA76GW-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | STA |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD106 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 7412 |
Format | Purified |
Immunogen | Human DS6 T cell line |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form of VCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis of subsequent cariovascular events following acute coronary syndromes. |
Alias Names | VCAM-1, INCAM-100 |
Previous Article No | AFC-4471-2, AFC-GA76GW-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 4-6 μg/ml; positive control: TNF-alpha activated HUVEC cells. Immunohistochemistry (frozen sections): Acetone fixation. ELISA: Capture mAb for soluble CD106. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody STA recognizes an extracellular epitope of CD106 antigen (VCAM-1), a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule. |
UniProt Number | P19320 |
Product Page Updated | 2024-01-03T12:18:20.318Z |