SDS extracted protein fraction of human myocardium_x000D_
Cytoskeleton: Actin System, Cytoskeleton: Actin System (Veterinary), Cytoskeleton: Actin System (Rodent), Cytoskeleton: Actin System (Human)
Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton. _x000D_
Purified by protein-A affinity chromatography
> 95% (by SDS-PAGE)
human, monkey, mouse, rat, dog/canine, cat/feline, rabbit, chicken
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells._x000D_
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