Article No
AFC-0HZCIT-0.1
Application | WB, IHC, ICC |
Article No | AFC-0HZCIT-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | HHF35 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Actin (muscle) Purified |
Supplier | Nordic Biosite |
Format | Purified |
Immunogen | SDS extracted protein fraction of human myocardium |
Isotype | IgG1 |
Keywords | Cytoskeleton: Actin System, Cytoskeleton: Actin System (Veterinary), Cytoskeleton: Actin System (Rodent), Cytoskeleton: Actin System (Human) |
Notes | Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton. |
Alias Names | Muscle specific actin |
Previous Article No | AFC-4468-2, AFC-0HZCIT-0.1 |
Product Type | Antibodies Primary |
Protocol | Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Cell Proliferation |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human, chicken, mouse, monkey, dog/canine, rat, rabbit, cat/feline |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells. |
Product Page Updated | 2024-01-03T12:18:20.318Z |