Article No
AFC-GUNICZ-0.1
Application | WB |
Article No | AFC-GUNICZ-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | EM-20 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD230 / Prion Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 5621 |
Format | Purified |
Immunogen | Recombinant human prion protein |
Isotype | IgG2a |
Keywords | CD and Related Antigens (Human), Neurobiology (Human) |
Notes | CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K. |
Alias Names | Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC |
Previous Article No | AFC-4465-2, AFC-GUNICZ-0.1 |
Product Type | Antibodies Primary |
Protocol | Western blotting: Recommended dilution: 0.5 μg/ml, non-reducing conditions are essential. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein. |
UniProt Number | P04156 |
Product Page Updated | 2024-01-03T12:18:20.318Z |