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Anti-Hu CD324 Purified

Antibodies Primary

Make a customized order

Article No

AFC-SF53ME-0.1

Species Reactivity

human

Size

0.1 mg

Clone

67A4

Source / Host

mouse

Application

FC, IP, WB, IHC, ICC

Article No

AFC-SF53ME-0.1

Species Reactivity

human

Size

0.1 mg

Clone

67A4

Source / Host

mouse

Application

FC, IP, WB, IHC, ICC

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB, IHC, ICC
Article No	AFC-SF53ME-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	67A4
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD324 Purified
Supplier	Nordic Biosite
Entrez Gene ID	999
Format	Purified
Immunogen	T-47D cells
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
Alias Names	Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1
Previous Article No	AFC-4461-2, AFC-SF53ME-0.1
Product Type	Antibodies Primary
Protocol	Immunohistochemistry (frozen sections): Recommended dilution: 4-8 μg/ml; positive tissue: tonsil. Flow cytometry: Recommended dilution: 5-10 µg/ml
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
UniProt Number	P12830
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Servet-Delprat C, Vidalain PO, Bausinger H, Manié S, Le Deist F, Azocar O, Hanau D, Fischer A, Rabourdin-Combe C: Measles virus induces abnormal differentiation of CD40 ligand-activated human dendritic cells. J Immunol. 2000 Feb 15;164(4):1753-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/10657621?itool=,*Furio L, Guezennec A, Ducarre B, Guesnet J, Peguet-Navarro J: Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells. Eur J Dermatol. 2008 Mar-Apr;18(2):141-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18424372?itool=,*Kutlesa S, Wessels JT, Speiser A, Steiert I, Müller CA, Klein G: E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation. J Cell Sci. 2002 Dec 1;115(Pt 23):4505-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/12414996?itool=,*Caberg JH, Hubert PM, Begon DY, Herfs MF, Roncarati PJ, Boniver JJ, Delvenne PO: Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes. Carcinogenesis. 2008 Jul;29(7):1441-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18566017?itool=,*Lin JC, Liao SK, Lee EH, Hung MS, Sayion Y, Chen HC, Kang CC, Huang LS, Cherng JM. Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome. J Biomed Sci. 2009 Nov 24;16:105., URL: http://www.ncbi.nlm.nih.gov/pubmed/19930697?itool=,*Furio L, Guezennec A, Ducarre B, Guesnet J, Peguet-Navarro J: Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells. Eur J Dermatol. 2008 Mar-Apr;18(2):141-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18424372?itool,*Armeanu S, Bühring HJ, Reuss-Borst M, Müller CA, Klein G: E-cadherin is functionally involved in the maturation of the erythroid lineage. J Cell Biol. 1995 Oct;131(1):243-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/7559781?itool=,*Bühring HJ, Müller T, Herbst R, Cole S, Rappold I, Schuller W, Zhu X, Fritzsch U, Faul C, Armeanu S, Ullrich A, Klein G, Schmidt H: The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages. Leukemia. 1996 Jan;10(1):106-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/8558914?itool=,*Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T: A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J Invest Dermatol. 2002 Oct;119(4):842-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12406329?itool,*Servet-Delprat C, Vidalain PO, Bausinger H, Manié S, Le Deist F, Azocar O, Hanau D, Fischer A, Rabourdin-Combe C: Measles virus induces abnormal differentiation of CD40 ligand-activated human dendritic cells. J Immunol. 2000 Feb 15;164(4):1753-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/10657621?itool,*Robertson H, Ali S, McDonnell BJ, Burt AD, Kirby JA: Chronic renal allograft dysfunction: the role of T cell-mediated tubular epithelial to mesenchymal cell transition. J Am Soc Nephrol. 2004 Feb;15(2):390-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/14747385?itool,*Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T: A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J Invest Dermatol. 2002 Oct;119(4):842-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/12406329?itool=,,*Pece S, Gutkind JS: Signaling from E-cadherins to the MAPK pathway by the recruitment and activation of epidermal growth factor receptors upon cell-cell contact formation. J Biol Chem. 2000 Dec 29;275(52):41227-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/10969083?itool,*Pece S, Chiariello M, Murga C, Gutkind JS: Activation of the protein kinase Akt/PKB by the formation of E-cadherin-mediated cell-cell junctions. Evidence for the association of phosphatidylinositol 3-kinase with the E-cadherin adhesion complex. J Biol Chem. 1999 Jul 2;274(27):19347-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/10383446?itool,*Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991 Mar 22;251(5000):1451-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2006419?itool=,,

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