Article No
AFC-OGNWBT-0.1
Application | FC, IP, IHC, ICC |
Article No | AFC-OGNWBT-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 53-6.7 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Ms CD8a Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 12525 |
Format | Purified |
Immunogen | Murine spleen cells |
Isotype | IgG2a k |
Keywords | CD and Related Antigens & MHC (Rodent) |
Notes | The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
Alias Names | Ly-2, Ly-B, Ly-35, Lyt-2, BB154331 |
Previous Article No | AFC-4452-2, AFC-OGNWBT-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1.5 μg/ml. Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred. |
Purification | Purified by protein-G affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | mouse |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen). |
UniProt Number | P01731 |
Product Page Updated | 2024-01-03T12:18:20.318Z |