Article No
AFC-GB8DSS-0.1
Application | FC, IHC |
Article No | AFC-GB8DSS-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | HM57 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD79a Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 973 |
Format | Purified |
Immunogen | Synthetic peptide corresponding to amino acids 202-216 of human CD79a |
Isotype | IgG1 |
Keywords | CD and Related Antigens & MHC (Rodent), CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling. |
Alias Names | BCR alpha, Ig-alpha, MB-1, IGA |
Previous Article No | AFC-4419-2, AFC-GB8DSS-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 5 μg/ml. Intracellular staining.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | chicken, rabbit, rodent, guinea pig, horse/equine, cow/bovine, pig/porcine, mouse, rat, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms. |
UniProt Number | P11912 |
Product Page Updated | 2024-01-03T12:18:20.318Z |