Article No
AFC-4FEMDD-0.1
Application | WB, IHC |
Article No | AFC-4FEMDD-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | DE-K13 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Cytokeratin 10/13 Purified |
Supplier | Nordic Biosite |
Format | Purified |
Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium. |
Isotype | IgG2a |
Keywords | Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments |
Notes | Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins). |
Alias Names | K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13 |
Previous Article No | AFC-4380-2, AFC-4FEMDD-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections): Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Cell Proliferation |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | cat/feline, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections. |
Product Page Updated | 2024-01-03T12:18:20.318Z |