Article No
AFC-MXZ4U2-0.1
Application | FC, IP, WB, IHC |
Article No | AFC-MXZ4U2-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 9E10 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-c-Myc tag Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 4609 |
Format | Purified |
Immunogen | Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc. |
Isotype | IgG1 |
Keywords | Epitope Tags |
Notes | The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells. |
Alias Names | bHLH, MRTL, MYCC |
Previous Article No | AFC-4344-2, AFC-MXZ4U2-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive tissue: perfused brain sections, liver, spleen. Immunoprecipitation: Recommended dilution: 1-5 μg/ml; this antibody is not suitable for immunoprecipitation of native c-Myc protein. Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 0,5-2 μg/ml; positive control: c-Myc tagged protein. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody 9E10 can be used to detect the c-Myc tag. |
UniProt Number | P01106 |
Product Page Updated | 2024-01-03T12:18:20.318Z |