Article No
AFC-Q44OCE-0.1
Application | FC, IHC, AGG |
Article No | AFC-Q44OCE-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | HIR2 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD235ab Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 2993 |
Format | Purified |
Immunogen | Synthetic peptide (Human, N-terminal) |
Isotype | IgG2b |
Keywords | CD and Related Antigens (Human) |
Notes | CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin. |
Alias Names | Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group |
Previous Article No | AFC-4335-2, AFC-Q44OCE-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-4 µg/ml |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody HIR2 recognizes the N-terminal (extracellular) portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. |
UniProt Number | P02724 |
Product Page Updated | 2024-01-03T12:18:20.318Z |