Article No
AFC-Y9CETO-0.1
Specifications
| Application | WB, IHC |
| Article No | AFC-Y9CETO-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | PAb (410) |
| Clone Type | polyclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-LIME1 Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 54923 |
| Format | Purified |
| Immunogen | Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME. |
| Keywords | Adaptor Proteins (Human), Adaptor Proteins |
| Notes | LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. |
| Alias Names | LITA1 |
| Previous Article No | AFC-4328-2, AFC-Y9CETO-0.1 |
| Product Type | Antibodies Primary |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells. |
| UniProt Number | Q9H400 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
