PAG / Cbp

Article No AFC-4327-2
Product Type Antibodies Primary
Research Area Immunology
Application IP, WB, IHC
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone Polyclonal
Clone Type polyclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Rabbit Polyclonal to PAG / Cbp
Supplier BioSite Flow
Immunogen Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Keywords Adaptor Proteins (Rodent), Adaptor Proteins (Human), Adaptor Proteins
Notes PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Purification Purified from rabbit serum by precipitation methods
Purity > 95% (by SDS-PAGE)
Species Reactivity mouse, human
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Technical Specifications The polyclonal antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Product Page Updated 2019-05-20T06:41:23.567Z

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Species reactivity:

mouse, human mouse human