Article No
AFC-52SN8M-0.1
Application | FC |
Article No | AFC-52SN8M-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-168 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD16 Purified |
Supplier | Nordic Biosite |
Format | Purified |
Immunogen | Human granulocytes |
Isotype | IgM |
Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype. |
Alias Names | FcgammaRIII, IGFR3, FCRIII |
Previous Article No | AFC-4304-2, AFC-52SN8M-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | monkey, human, pig/porcine |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
Technical Specifications | The antibody MEM-168 reacts with an extracellular epitope of CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils). |
Product Page Updated | 2024-01-03T12:18:20.318Z |