Article No
AFC-ZL9O70-0.1
Specifications
| Application | WB, IP, ICC |
| Article No | AFC-ZL9O70-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | CSK-04 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Anti-CSK Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 1445 |
| Format | Purified |
| Immunogen | Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450. |
| Isotype | IgG1 |
| Keywords | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) |
| Notes | C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk. |
| Alias Names | C-terminal Src kinase |
| Previous Article No | AFC-4300-2, AFC-ZL9O70-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line. Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions. Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 ml |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase. |
| UniProt Number | P41240 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
