Article No
AFC-99VMHY-0.1
Anti-GAPDHS Purified
Anti-GAPDHS Purified
Antibodies Primary
Specifications
| Application | WB, ICC, FC |
| Article No | AFC-99VMHY-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | Hs-8 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-GAPDHS Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 26330 |
| Format | Purified |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Isotype | IgM |
| Keywords | Fertility & Reproduction (Veterinary), Fertility & Reproduction (Human), Fertility & Reproduction |
| Notes | GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Alias Names | SGAPDH, HSD-35 |
| Previous Article No | AFC-4236-2, AFC-99VMHY-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining. |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | pig/porcine, human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Technical Specifications | The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein. |
| UniProt Number | O14556 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
