Vimentin

Article No AFC-4200-2
Product Type Antibodies Primary
Research Area Cell Proliferation
Application WB, ICC
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone VI-01
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Mouse Monoclonal to Vimentin
Supplier BioSite Flow
Immunogen Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Isotype IgM
Keywords Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments
Notes Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP)._x000D_ Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin._x000D_ Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Purification Purified by precipitation and chromatography
Purity > 95% (by SDS-PAGE)
Species Reactivity other mammals
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Technical Specifications The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.<br>_x000D_
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

other mammals

Suggested protocols:

Western Blot

Western Blot