Article No
AFC-90GIQY-0.1
Anti-Hu beta2-Microglobulin Purified
Anti-Hu beta2-Microglobulin Purified
Antibodies Primary
Specifications
| Application | FC, IP, WB, IHC, ICC, ELISA, RIA |
| Article No | AFC-90GIQY-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | B2M-01 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu beta2-Microglobulin Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 567 |
| Format | Purified |
| Immunogen | Purified human beta2-microglobulin |
| Isotype | IgG2a |
| Keywords | Serum & Plasma Proteins (Human), MHC (Major Histocompatibility Complex) and Related Antigens (Human) |
| Notes | Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. |
| Alias Names | B2M |
| Previous Article No | AFC-4186-1, AFC-90GIQY-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
| UniProt Number | P61769 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
