Article No
AFC-KTXBOO-0.1
Application | FC, IP, WB, IHC |
Article No | AFC-KTXBOO-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-53 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD53 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 963 |
Format | Purified |
Immunogen | Leukocytes of pacient suffering from a LGL-type leukemia. |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression. |
Alias Names | Tetraspanin-25, MOX44, TSPAN25 |
Previous Article No | AFC-4177-1, AFC-KTXBOO-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Flow cytometry: Recommended dilution: 4 μg/ml. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). |
UniProt Number | P19397 |
Product Page Updated | 2024-01-03T12:18:20.318Z |