Article No
AFC-SEFXGX-0.1
Application | FC, IP, WB, IHC |
Article No | AFC-SEFXGX-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-55 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD45RB Purified |
Supplier | Nordic Biosite |
Format | Purified |
Immunogen | Human thymocytes and T lymphocytes. |
Isotype | IgG1 |
Keywords | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells. |
Alias Names | PTPRCR, T200RB |
Previous Article No | AFC-4174-1, AFC-SEFXGX-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 2 μg/ml. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | monkey, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-55 recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. |
Product Page Updated | 2024-01-03T12:18:20.318Z |