Article No
AFC-69KD4S-0.1
Application | FC, IP, WB, IHC |
Article No | AFC-69KD4S-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-48 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD18 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 3689 |
Format | Purified |
Immunogen | Leukocytes of a patient suffering from a LGL-type leukemia. |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. |
Alias Names | Integrin beta2, Complement receptor C3 subunit beta, ITGB2 |
Previous Article No | AFC-4165-2, AFC-69KD4S-0.1 |
Product Type | Antibodies Primary |
Protocol | Western blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia. |
Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. |
UniProt Number | P05107 |
Product Page Updated | 2024-01-03T12:18:20.318Z |