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Anti-Hu CD18 Purified

Antibodies Primary

Make a customized order

Article No

AFC-69KD4S-0.1

Species Reactivity

human

Size

0.1 mg

Clone

MEM-48

Source / Host

mouse

Application

FC, IP, WB, IHC

Article No

AFC-69KD4S-0.1

Species Reactivity

human

Size

0.1 mg

Clone

MEM-48

Source / Host

mouse

Application

FC, IP, WB, IHC

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB, IHC
Article No	AFC-69KD4S-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MEM-48
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD18 Purified
Supplier	Nordic Biosite
Entrez Gene ID	3689
Format	Purified
Immunogen	Leukocytes of a patient suffering from a LGL-type leukemia.
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Alias Names	Integrin beta2, Complement receptor C3 subunit beta, ITGB2
Previous Article No	AFC-4165-2, AFC-69KD4S-0.1
Product Type	Antibodies Primary
Protocol	Western blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.
Purification	Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
UniProt Number	P05107
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69., URL: http://www.ncbi.nlm.nih.gov/pubmed/18922855 ,*Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/9376602 ,*Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/10233903?itool=,*Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/10820275?itool=,*Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1715364?dopt=A,*Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217., URL: ,*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/1971601?dopt=A,,*Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10., URL: https://pubmed.ncbi.nlm.nih.gov/17698568/,*Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15., URL: https://pubmed.ncbi.nlm.nih.gov/17069006/,*Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45., URL: https://pubmed.ncbi.nlm.nih.gov/15485828/,*Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706., URL: https://pubmed.ncbi.nlm.nih.gov/14634077/,*Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9., URL: https://pubmed.ncbi.nlm.nih.gov/9645379/,*Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10964661?dopt=,*Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70., URL: https://pubmed.ncbi.nlm.nih.gov/8774366/,,

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