Article No
AFC-VQYCFR-0.1
Anti-Cytokeratin 18 Purified
Anti-Cytokeratin 18 Purified
Antibodies Primary
Specifications
| Application | IP, WB, IHC, ICC, ELISA |
| Article No | AFC-VQYCFR-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | DA-7 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Cytokeratin 18 Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3875 |
| Format | Purified |
| Immunogen | Human breast carcinoma cell line PMC-42. |
| Isotype | IgG1 |
| Keywords | Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments |
| Notes | Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins). |
| Alias Names | K18, CK18, CYK18, KRT18 |
| Previous Article No | AFC-4125-1, AFC-VQYCFR-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100). |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody DA-7 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues. |
| UniProt Number | P05783 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
