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Anti-Cytokeratins Purified

Antibodies Primary

Make a customized order

Article No

AFC-JQXVW6-0.1

Species Reactivity

mammals

Size

0.1 mg

Clone

C-11

Source / Host

mouse

Application

FC, WB, IP, IHC, ICC

Article No

AFC-JQXVW6-0.1

Species Reactivity

mammals

Size

0.1 mg

Clone

C-11

Source / Host

mouse

Application

FC, WB, IP, IHC, ICC

Specifications
Suggested protocols
References

Specifications

Application	FC, WB, IP, IHC, ICC
Article No	AFC-JQXVW6-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	C-11
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Cytokeratins Purified
Supplier	Nordic Biosite
Format	Purified
Immunogen	Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Isotype	IgG1
Keywords	Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments
Notes	Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Alias Names	cytokeratin, CYK, CK, KRT
Previous Article No	AFC-4123-1, AFC-JQXVW6-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Cell Proliferation
Size	0.1 mg
Source / Host	mouse
Species Reactivity	mammals
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26., URL: https://pubmed.ncbi.nlm.nih.gov/9821208/,*Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/16316332?dopt=,*Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834,*Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228?dopt=A,*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A,,,

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