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Anti-Ms MHC Class II Purified Azide Free

Antibodies Primary

Make a customized order

Article No

AFC-Q3YHCA-0.1

Species Reactivity

mouse

Size

0.1 mg

Clone

M5/114

Source / Host

mouse

Application

FC, IP, WB, IHC

Article No

AFC-Q3YHCA-0.1

Species Reactivity

mouse

Size

0.1 mg

Clone

M5/114

Source / Host

mouse

Application

FC, IP, WB, IHC

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB, IHC
Article No	AFC-Q3YHCA-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	M5/114
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Ms MHC Class II Purified Azide Free
Supplier	Nordic Biosite
Format	Purified Azide Free
Immunogen	Activated C57BL/6 mouse spleen cells
Isotype	IgG2b
Keywords	CD and Related Antigens & MHC (Rodent)
Notes	MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Previous Article No	AFC-4111-2, AFC-Q3YHCA-0.1
Product Type	Antibodies Primary
Protocol	Functional application: Blocking of T cell proliferative responses. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Purification	Purified by protein-G affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	mouse
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4
Technical Specifications	The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8(2):143-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9491996?itool=,*Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec;16(12):3661-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16221866?itool,*Kuwano Y, Pražma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug;19(8):977-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/17804692?itool,*Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1;174(3):1205-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/15661874?itool,*Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1;155(1):53-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/11581285?itool,*De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct;108 ( Pt 10):3219-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/7593283?itool=,*Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 26;72(4):635-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/7679955?itool=,*Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127(6):2488-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6170707?itool=,,,

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