Article No
AFC-F3OK9U-0.1
Anti-Hu CD54 Purified Azide Free
Anti-Hu CD54 Purified Azide Free
Antibodies Primary
Specifications
| Application | FC, WB, IHC, ICC |
| Article No | AFC-F3OK9U-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 1H4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD54 Purified Azide Free |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3383 |
| Format | Purified Azide Free |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Isotype | IgG2b |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| Alias Names | ICAM-1, BB2, P3.58 |
| Previous Article No | AFC-4099-2, AFC-F3OK9U-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 μg/ml. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Technical Specifications | The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. |
| UniProt Number | P05362 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
