CD11a

Article No AFC-4095-2
Product Type Antibodies Primary
Research Area Immunology
Application FA, FC, IP
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MEM-83
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Mouse Monoclonal to CD11a / LFA-1 alpha chain
Supplier BioSite Flow
Immunogen Human peripheral blood lymphocytes
Isotype IgG1
Keywords CD and Related Antigens (Human)
Notes CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Purification Purified by protein-A affinity chromatography
Purity > 95% (by SDS-PAGE)
Species Reactivity human
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized.
Technical Specifications The antibody MEM-83 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. <br>_x000D_ <b>HLDA IV; WS Code N 211</b>
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

human human

Suggested protocols:

Immunoprecipitation

Immunoprecipitation