Transferrin

Article No AFC-4088-2
Product Type Antibodies Primary
Research Area Cell Proliferation
Application WB, IHC-P, ICC, ELISA, RIA, FA
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone HTF-14
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Mouse Monoclonal to Transferrin
Supplier BioSite Flow
Immunogen Purified porcine transferrin.
Isotype IgG1
Keywords Endocytosis (Veterinary), Endocytosis (Human), Serum & Plasma Proteins (Veterinary), Serum & Plasma Proteins (Human)
Notes Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. _x000D_ Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division._x000D_ _x000D_ _x000D_
Purification Purified by precipitation and chromatography
Purity > 95% (by SDS-PAGE)
Species Reactivity human, rabbit, pig/porcine
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized.
Technical Specifications The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+._x000D_ _x000D_
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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Species reactivity:

human, rabbit, pig/porcine human rabbit

Suggested protocols:

Western Blot

Western Blot