Article No
AFC-02M9NX-0.1
Anti-Transferrin Purified Azide Free
Anti-Transferrin Purified Azide Free
Antibodies Primary
Specifications
| Application | WB, IHC, ICC, ELISA, RIA |
| Article No | AFC-02M9NX-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | HTF-14 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Transferrin Purified Azide Free |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 7018 |
| Format | Purified Azide Free |
| Immunogen | Purified porcine transferrin. |
| Isotype | IgG1 |
| Keywords | Endocytosis (Veterinary), Endocytosis (Human), Serum & Plasma Proteins (Veterinary), Serum & Plasma Proteins (Human) |
| Notes | Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division. |
| Alias Names | TFQTL1, PRO1557, PRO2086 |
| Previous Article No | AFC-4088-2, AFC-02M9NX-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/ml. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human, pig/porcine, rabbit |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Technical Specifications | The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. |
| UniProt Number | P02787 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
