Article No
AFC-R69N1C-100
Specifications
| Application | FC |
| Article No | AFC-R69N1C-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | TU27 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD122 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3560 |
| Format | PE |
| Immunogen | TL-Mor cell line |
| Isotype | IgG1 k |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex. |
| Alias Names | IL2RB, IL15RB, P70-75 |
| Previous Article No | AFC-338A5C |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation. |
| UniProt Number | P14784 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
