Article No
AFC-1S5WO0-100
Anti-Hu CD49d APC-Cy™7
Anti-Hu CD49d APC-Cy™7
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-1S5WO0-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 9F10 |
| Clone Type | monoclonal |
| Conjugation | APC/Cy7 |
| Description | Anti-Hu CD49d APC-Cy™7 |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3676 |
| Format | APC-Cy™7 |
| Isotype | IgG1 k |
| Notes | CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins. |
| Alias Names | ITGA4, VLA-4 alpha |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | sheep/ovine, cat/feline, horse/equine, dog/canine, cow/bovine, monkey, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets. |
| UniProt Number | P13612 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
