Article No
AFC-0XPD7L-100
Anti-Hu CD274 PE-Cy™7
Anti-Hu CD274 PE-Cy™7
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-0XPD7L-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 29E.2A3 |
| Clone Type | monoclonal |
| Conjugation | PE/Cy7 |
| Description | Anti-Hu CD274 PE-Cy™7 |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 29126 |
| Format | PE-Cy™7 |
| Immunogen | Full length human CD274 |
| Isotype | IgG2b k |
| Notes | CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely. |
| Alias Names | B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | monkey, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas. |
| UniProt Number | Q9NZQ7 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
