Article No
AC-0237
Application | IHC |
Article No | AC-0237 |
Country Availability | SE, FI, DK, NO, IS, FO, GL |
Clone | EP268 |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | SOX10 |
Supplier | Cell Marque (Epitomics) |
Format | Concentrate |
Notes | SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. During development, SOX10 first appears in the forming neural crest and continues to be expressed in Schwann cells. It is important for differentiation, maturation and maintenance of Schwann cells and melanocytes. In normal tissues, SOX10 is expressed in Schwann cells and glial cells in the nervous system. It is also detected in melanocytes and epithelial cells of salivary gland and mammary gland. In tumor tissues, SOX10 labels melanoma and the tumor of neural crest origin. A recent study reported the expression of SOX10 in basal-like, unclassified triple-negative breast carcinoma. Thus, breast carcinoma must be considered in the differential diagnosis of melanoma for a SOX10-positive metastatic malignant neoplasm. |
Product Type | Antibodies Primary |
Protocol | IHC: 1:100-1:2001. Pretreatment: Epitope retrieval using citrate buffer (catalog #: SP-0001) with a pressure cooker.2. Endogenous peroxidase block: Block for 10 minutes at room temperature using peroxidase solution (catalog #: SP-0002).3. Protein block: Block for 10 minutes at room temperature using blocking solution (catalog #: SP-0003).4. Primary antibody: Incubate for 30 minutes.5. Detection: Follow instructions from the selected detection system (EpiPrecision™, a Biotin Streptavitin-HRP Detection, catalog #: DK-0001, 0003, or EpiVision™, a Rabbit Polymer Detection, catalog # DK-0002, 0004).The antibody dilution and protocol may vary depending on the specimen preparation and specific application. Optimal conditions should be determined by the individual laboratory. |
Research Area | Clinical Diagnostic |
Size | 1 mL |
Source / Host | rabbit |
Species Reactivity | human |
Storage | Store at 2-8 °C. Do not use after expiration date indicated on vial. End user must validate any storage conditions other than those specified. |
Substrate / Buffer | Antibody to SOX10 is affinity purified and diluted in 10 mM Phosphate buffered saline (PBS), pH 7.2 containing 1% bovine serum albumin (BSA) and 0.09% sodium azide (NaN3). |
Product Page Updated | 2023-10-02T09:48:37.458Z |