Article No
ABD-1310
Application | Labeling, IHC, WB |
Article No | ABD-1310 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Conjugation | PE |
Description | Buccutite™ Rapid PE Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction* |
Supplier | AAT Bioquest |
Product Type | Sample Preparation |
Shipping Information | BLUE ICE |
Size | 2 Labelings |
Stability | 6 months |
Storage | r/l |
Substrate / Buffer | DMSO |
Technical Specifications | PE is an orange fluorescent protein which has an excitation wavelength of 565nm and an emission wavelength of 575 nm. ReadiLink PE Conjugation Kit provides a robust and convenient method to conjugate your antibodies with PE. The kit includes an activated PE and reaction buffer. The entire process only require two simple mixings, and no further purification is required. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of RPE. The best ratio for any new antibody reagent must be determined by experimentation but 50-60 ug of IgG antibody for every 100 ug of RPE usually gives optimal results. 60 ug of antibody approximately corresponds to an Antibody:RPE molar ratio of 1:1. |
Product Page Updated | 2023-03-15T11:57:07.008Z |