14-3-3 epsilon

Article No AAT-90445-1
Product Type Antibodies Primary
Research Area Neuroscience
Application ELISA, WB, FC, ICC
Size 50ul

Specifications

NCBI Number NP_006752
Country Availability all
BioSite Brand BioSite
Clone Anti-human 14-3-3 epsilon mAb, clone AT4F8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 epsiilon protein.
Clone Type monoclonal
Concentration 1 mg/ml
Description 14-3-3 epsilon antibody
Supplier BioSite
Immunogen Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli
Isotype IgG2b k
Notes The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
Alias Names Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide, YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14-3-3 epsilon, Tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.
Protocol The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Purification By protein-A affinity chromatography
Source / Host mouse
Species Reactivity human
Storage Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Substrate / Buffer Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Technical Specifications The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Shipping info

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Source/Host:

mouse mouse

Species reactivity:

human human

Suggested protocols:

Western Blot

Western Blot