Article No
A68315-100
ESR1 Polyclonal Antibody
ESR1 Polyclonal Antibody
Antibodies Primary
Specifications
| Application | ELISA, WB, IHC, ChIP |
| Article No | A68315-100 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | ESR1 Polyclonal Antibody |
| Supplier | Epigentek |
| Immunogen | Recombinant Human Estrogen receptor protein (10-591AA) |
| Isotype | IgG |
| Keywords | Transcription Factors |
| Notes | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1. _x000D_ |
| Alias Names | ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1 |
| Product Type | Antibodies Primary |
| Purity | Protein G purified |
| Research area | Cell Proliferation |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | human, rat |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze. |
| Substrate / Buffer | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4 |
| UniProt Number | P03372 |
| Product Page Updated | 2024-02-13T10:42:34.821Z |
