Article No
A-4032-050
Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody
Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody
Antibodies Primary
Specifications
| Application | WB |
| Article No | A-4032-050 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody |
| Supplier | Epigentek |
| Format | Affinity Purified |
| Gene Symbol | H3K4me2 |
| Immunogen | Synthetic Peptide |
| Isotype | IgG |
| Keywords | Histone Methylation & Demethylation |
| Notes | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| Alias Names | H3K4me2 antibody; H3K4m2 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3 |
| Product Type | Antibodies Primary |
| Purity | Affinity Purified |
| Research area | Epigenetics |
| Shipping Information | Shipped at 4°C. Aliquot store at -20°C . Avoid repeated freeze / thaw cycles. |
| Size | 50 µl |
| Source / Host | rabbit |
| Species Reactivity | mouse, rat, human |
| Storage | Shipped at 4°C. Aliquot store at -20°C . Avoid repeated freeze / thaw cycles. |
| Substrate / Buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| UniProt Number | P68431/Q71DI3/P84243 |
| Product Page Updated | 2024-02-13T10:42:34.821Z |
