Article No
713-185-147
Application | ICC, IHC |
Article No | 713-185-147 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone Type | polyclonal |
Conjugation | Gold |
Description | 4 nm Colloidal Gold-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (LM Grade) |
Recommended Dilution | ICC 1:20-1:200, IHC 1:20-1:200 |
Supplier | Jackson ImmunoResearch |
Format | Affinity Purified |
Immunogen | IgG (H+L) |
Isotype | IgG |
Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species. |
Product Type | Antibodies Secondary |
Protocol | Histo-/Cyto-Chemistry:- 1:20-1:200 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Purity | AffiniPure |
Shipping Information | RT |
Size | 0.5 ml |
Source / Host | donkey |
Species Reactivity | sheep/ovine |
Storage | 4°C |
Substrate / Buffer | 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5 |
Product Page Updated | 2023-12-29T14:15:50.354Z |