Article No
709-056-073
Alkaline Phosphatase-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Human IgM, Fc5µ Fragment Specific (min X Bov,Hrs Sr Prot)
Alkaline Phosphatase-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Human IgM, Fc5µ Fragment Specific (min X Bov,Hrs Sr Prot)
Antibodies Secondary
Specifications
| Application | ELISA, WB |
| Article No | 709-056-073 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | AP |
| Description | Alkaline Phosphatase-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Human IgM, Fc5µ Fragment Specific (min X Bov,Hrs Sr Prot) |
| Recommended Dilution | ELISA 1:5,000-1:50,000, WB 1:5,000-1:50,000 |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgM, Fc5µ fragment |
| Isotype | IgM |
| Notes | F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine and horse serum proteins, but it may cross-react with IgM from other species. |
| Product Type | Antibodies Secondary |
| Protocol | ELISA:- 1:5,000-1:50,000Western Blot:- 1:5,000-1:50,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.5 ml |
| Source / Host | donkey |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
