Article No
515-055-003
Alkaline Phosphatase-conjugated AffiniPure Sheep Anti-Mouse IgG (H+L)
Alkaline Phosphatase-conjugated AffiniPure Sheep Anti-Mouse IgG (H+L)
Antibodies Secondary
Specifications
| Application | ELISA, WB |
| Article No | 515-055-003 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | AP |
| Description | Alkaline Phosphatase-conjugated AffiniPure Sheep Anti-Mouse IgG (H+L) |
| Recommended Dilution | ELISA 1:5,000-1:50,000, WB 1:5,000-1:50,000 |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG (H+L) |
| Isotype | IgG |
| Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | ELISA:- 1:5,000-1:50,000Western Blot:- 1:5,000-1:50,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 1 ml |
| Source / Host | sheep/ovine |
| Species Reactivity | mouse |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
