Article No
36-3297-20
p53 Tumor Suppressor Protein
Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: DO-1)
Antibodies Primary
Specifications
| Application | FC, IF, IHC, WB |
| Article No | 36-3297-20 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | DO-1 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: DO-1) |
| Supplier | Abeomics |
| Entrez Gene ID | 7157 |
| Gene Symbol | TP53 |
| Immunogen | Recombinant human wild type p53 protein expressed in E. coli. |
| Isotype | IgG2a k |
| Notes | Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 20-25) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. |
| Alias Names | Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) |
| Shipping Information | Blue Ice |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C, -80°C |
| Substrate / Buffer | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| UniProt Number | P04637 |
| Product Page Updated | 2024-02-06T07:35:53.072Z |
