Article No
333-A8750-25mg
Specifications
| CAS No | 219138-21-3 |
| MW | 638.62 |
| Article No | 333-A8750-25mg |
| Country Availability | SE, FI, DK, NO, IS, EE, FO, GL |
| Description | Ac-IETD-pNA |
| Supplier | APExBIO |
| Notes | Ac-IETD-pNA is a colorimetric substrate for caspase-8 and other similar cysteine proteases (caspase-6, caspase-10 and granzyme B). It recognizes the amino acid sequence IETD which is based on caspase-8 cleavage site in Caspase-3 precursor. During apoptosis, caspase 8 cleaves the substrate and releases pNA, thus the caspase activity can be calculated by the spectrophotometric detection of pNA at 405nm. Ac-IETD-pNA can be used to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation. References:1. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)2. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994)3. Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994) |
| Molecular Formula | C27H38N6O12 |
| Previous Article No | 333-A8750-25MG |
| Product Type | Assay & Detection |
| Purity | 99.75% |
| Shipping Information | BLUE ICE |
| Size | 25 mg |
| Solubility | ≥63.9 mg/mL in DMSO; ≥14.03 mg/mL in H2O; ≥5.99 mg/mL in EtOH with ultrasonic |
| Storage | -20°C |
| Product Page Updated | 2024-03-05T10:50:49.934Z |