Article No
318-GP26
Specifications
| Application | IHC, WB |
| Article No | 318-GP26 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Anti-Cingulin, guinea pig pab |
| Supplier | Progen |
| Immunogen | full length GST-fusion protein of human cingulin |
| Notes | The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). It is negative with endothelia of vessels and capillaries (e.g. in heart and brain). Polypeptide reacting: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein). Reactivity on cultured cell lines: Carcinoma cell lines of human(e.g. MCF-7, Caco-2, PLC, A-431), bovine (MDBK, BMGE) and canine (MDCK) origin. Positive control: total cell lysate of e.g. CaCo-2 cells. |
| Product Type | Antibodies Primary |
| Purification | stabilized antiserum |
| Size | 100 µL |
| Source / Host | guinea pig |
| Species Reactivity | cow/bovine, human, mouse, rabbit |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Substrate / Buffer | contains 0.09% sodium azide and 0.5% BSA |
| Technical Specifications | The anti-cingulin antibody reacts with a 150 kD polypeptide in tight junctions (zonula occludens) of polar epithelia and of stratified epithelia. |
| Product Page Updated | 2024-01-25T14:20:33.693Z |
