Article No
318-65158
Additional Information | negative with AAV4, 11, 12 |
Application | IHC, IP, WB |
Article No | 318-65158 |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone | B1 |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | anti-AAV VP1/VP2/VP3, B1, mouse mab, SN |
Supplier | Progen |
Immunogen | adeno-associated virus 2 capsid proteins and virus particles |
Isotype | IgG1 |
Notes | Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.Wobus CA, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol 74, 9281-9293 (2000). |
Product Type | Antibodies Primary |
Purification | hybridoma cell culture supernatant |
Size | 5 mL |
Source / Host | mouse |
Species Reactivity | virus |
Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
Substrate / Buffer | contains 0.09% sodium azide |
Technical Specifications | Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced degree with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction) |
Product Page Updated | 2024-01-25T14:16:46.985Z |