Article No
303-Z150M
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Application | WB, IP |
Article No | 303-Z150M |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone | 8C-1 |
Clone Type | monoclonal |
Concentration | See vial for concentration |
Conjugation | Unconjugated |
Description | Mouse anti Yeast GAL-4 DNA Binding Domain |
Supplier | Exalpha Biologicals |
Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells. |
Isotype | IgG1 |
Notes | Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription. |
Alias Names | Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast |
Product Type | Antibodies Primary |
Purification | Protein A/G Chromatography |
References | 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594. |
Research Area | Molecular Biology |
Shipping Information | Ship at ambient temperature, freeze upon arrival |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | yeast |
Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
Technical Specifications | Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 µg/ml. Optimal concentration should be evaluated by serial dilutions. |
Product Page Updated | 2019-01-09T10:09:28.847Z |
The delivery time for this item is approximately 5-8 business days.
If other deviations occur, this will be noted on the order confirmation or communicated via email.
Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.