Antibodies Primary

GAL-4 DNA Binding Domain

Mouse anti Yeast GAL-4 DNA Binding Domain

Article No




Species Reactivity



100 µg



Source / Host


Shipping Information

Ship at ambient temperature, freeze upon arrival

Product insert


Application WB, IP
Article No 303-Z150M
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone 8C-1
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Unconjugated
Description Mouse anti Yeast GAL-4 DNA Binding Domain
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
Isotype IgG1
Notes Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Alias Names Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.
Research Area Molecular Biology
Shipping Information Ship at ambient temperature, freeze upon arrival
Size 100 µg
Source / Host mouse
Species Reactivity yeast
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 µg/ml. Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2020-01-07T12:59:09.171Z

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