Article No
303-Z150M
Antibodies Primary
GAL-4 DNA Binding Domain
Mouse anti Yeast GAL-4 DNA Binding Domain
Product insert
Suggested protocols
Specifications
| Application | WB, IP |
| Article No | 303-Z150M |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 8C-1 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Description | Mouse anti Yeast GAL-4 DNA Binding Domain |
| Supplier | Exalpha Biologicals |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells. |
| Isotype | IgG1 |
| Notes | Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription. |
| Alias Names | Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast |
| Product Type | Antibodies Primary |
| Purification | Protein A/G Chromatography |
| References | 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594. |
| Research Area | Molecular Biology |
| Shipping Information | Ship at ambient temperature, freeze upon arrival |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | yeast |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
| Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
| Technical Specifications | Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 µg/ml. Optimal concentration should be evaluated by serial dilutions. |
| Product Page Updated | 2020-01-07T12:59:09.171Z |
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