Antibodies Primary

GAL-4 Activation Domain

NEW
Mouse anti Yeast GAL-4 Activation Domain

Article No

303-Z145M

Application

WB, IP

Species Reactivity

yeast

Size

100 µg

Clone

5C8-5

Source / Host

mouse

Shipping Information

Ship at ambient temperature, freeze upon arrival

Product insert

Specifications

Application WB, IP
Article No 303-Z145M
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone 5C8-5
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Unconjugated
Description Mouse anti Yeast GAL-4 Activation Domain
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
Isotype IgG1
Notes GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.
Research Area Molecular Biology
Shipping Information Ship at ambient temperature, freeze upon arrival
Size 100 µg
Source / Host mouse
Species Reactivity yeast
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications Antibody can be used for Western Blotting (5-10µg/ml). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2019-01-09T10:09:28.847Z

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