Antibodies Primary

M13 bacteriophage Biotinylated

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Mouse anti M13 bacteriophage (g3p), conjugated with Biotin

Article No

303-Z110M

Application

ELISA, WB, FC, IHC

Size

100 µg

Clone

E1

Source / Host

mouse

Shipping Information

Ship at ambient temperature, freeze upon arrival

Product insert

Suggested protocols

Specifications

Application ELISA, WB, FC, IHC
Article No 303-Z110M
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone E1
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Biotin
Description Mouse anti M13 bacteriophage (g3p), conjugated with Biotin
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
Isotype IgG3
Notes The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities._x000B_Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest._x000B_Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
Alias Names Coat protein A; g3p; Minor coat protein
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1- Van Wezenbeek P.M., Schoenmakers J.G.; Nucleotide sequence of the genes III, VI and I of bacteriophage M13; Nucleic Acids Res. 6:2799-2818(1979). 2- Van Wezenbeek P.M., Hulsebos T.J., Schoenmakers J.G.; Nucleotide sequence of the filamentous bacteriophage M13 genome: comparison with phage fd; Gene 11:129-148(1980). 3- Cleary J.M., Ray D.S.; Deletion analysis of the cloned replication origin region from bacteriophage M13; J. Virol. 40:197-203(1981). 4- Hines J.C., Ray D.S.; Construction and characterization of new coliphage M13 cloning vectors; Gene 11:207-218(1980). 5- Yanisch-Perron C., Vieira J., Messing J.; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors; Gene 33:103-119(1985). 6- Messing J.; New M13 Vectors for Cloning; Meth. Enzymol. 101:20 78(1983). 7- Sanger F., Nicklen S., Coulson A.R.; DNA sequencing with chain-terminating inhibitors; Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977). 8- Zoller M.J., Smith M.; Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors; Meth. Enzymol. 100:468-500(1983). 9- Hu N.T., Messing J.; The making of strand-specific M13 probes; Gene 17:271-277(1982). 10- Heidecker G., Messing J., Gronenborn B.; A versatile primer for DNA sequencing in the M13mp2 cloning system; Gene 10:69-73(1980). 11- Ebright R., Dong Q., Messing J.; Corrected nucleotide sequence of M13mp18 gene III; Gene 114:81-83(1992). 12- Hong G.F.; A method for sequencing single-stranded cloned DNA in both directions; Biosci. Rep. 1:243-252(1981). 13- Messing J.; Multipurpose cloning system based on the single-stranded DNA bacteriophage M13; Recombinant DNA Technical Bulletin NIH 2:43-48(1979).
Research Area Molecular Biology
Shipping Information Ship at ambient temperature, freeze upon arrival
Size 100 µg
Source / Host mouse
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications Antibody specific for the g3p protein. Antibody can be used for immunohistochemistry, Western blot (1-5 µg/ml), Flow cytometry (1 µg/106 cells) and ELISA. Optimal concentration should be evaluated by serial dilutions.
UniProt Number P69168 (M13 phage)
Product Page Updated 2019-01-09T10:09:28.847Z

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